蛋白质与罗丹明B相互作用的极谱分析

Electrochemical Study of Rhodamine B Interacting with Bovine Serum Albumin(BSA)

在pH5．0的六次甲基四胺缓冲液中，罗丹明B能够与蛋白质相互作用形成复合物。该复合物使罗丹明B在-0．87V处的极谱还原峰峰电流下降，在实验选定的最佳条件下，该峰峰电流的下降值同牛血清白蛋白（BSA）的含量在2．5～30mg／L范围内呈线性关系，检出限为1．3mg／L。该法已应用于血清样品中蛋白质的测定，分析结果满意。

Rhodamine B can react with bovine serum albumin to produce an electrochemically non-active supermolecular Rhodamine B BSA in the buffer solution of （CH2）6 N4 （pH 5.0）. Rhodamine B has welldefined second order derivative linear-sweep polarographic wave at 0. 87 V（vs. SCE）. Adding BSA into the Rhodamine B solution resulted in the decrease of the peak current but not change of peak potential. The decrease of the peak current is proportional to BSA concentration from 2. 5 to 30 mg/L, the limit of the detection is 1.3 mg/L, and the correlation coefficient is 0. 996, this method can be applied to determine BSA concentration.