兔舍中气载内毒素的测定

Detection of Airborne Endotoxin in Rabbit Houses

本试验采用ANDERSEN-6级和AGI-30（AGI=All Glass Impinger）空气微生物样品收集器分别对三个不同兔场环境中气载内毒素及革兰氏阴性菌含量进行了测定，并对二者在数量上的关系进行了探讨。此外，还对兔舍内环境中气载内毒素和气载革兰氏阴性菌的来源进行了初步分析。结果表明，兔舍内气载内毒素含量介于22～774EU／m^3空气之间；气载需氧革兰氏阴性菌含量在0．39～10．3×10^2CFU／m^3之间，没有检测出专性厌氧的革兰氏阴性活菌。在三个兔场中，革兰氏阴性菌含量与内毒素含量之间存在弱的正相关（rA=0．23；rB=0．29；rC=0．24），所以不能通过测定气载革兰氏阴性细菌含量来估计气载内毒素的含量。通过对气载内毒素来源的分析，可以推测饲料和粪便可能是其主要的来源。

In this study, the concentration of airborne endotoxin and airborne gram-negative bacteria were measured by the ANDERSEN six-stage sampler and AGI-30 （AGI= All Glass Impinger） sampler in three different rabbit houses. At the same time, were the quantitative relationship between the concentration of airborne endotoxin and airborne gram-negative bacteria. Moreover, the sources of airborne endotoxin and airborne gram-negative bacteria were investigated by detecting rabbits＂ feeding stuff and feces. The result showed that the concentration of airborne indotoxin and airborne gram-negative bacteria were 22 -774 EU/m^3 （EU=endotoxin units） and 0.39-10.3 × 10^2 CFU/m^3 （CFU=colony forming units） respectively. Obligate anaerobic airborne gram-negative bacteria were not detected in this experiment. Significant correlations were found between the concentration of airborne endotoxin and airborne gram-negative bacteria. However, these correlations were not very strong（rA=0.23; rB=0.29; rC=0.24）. So the total number of the airborne gram-negative bacteria should not be used for the estimation of the concentration of air- borne endotoxin. Moreover, by analyzing the concentration of endotoxin and gram-negative bacteria in feeding stuff and feces, it is deduced that the main source of airborne endotoxin and airborne gram-negative bacteria were feeding stuff and feces.