胶体染料试条法在血吸虫病诊断中的应用价值

Evaluation of DDIA Method in Diagnosis of Patients With Schistosomiasis

目的 建立一种简便、快速、准确地诊断血吸虫病的方法，并与金标准COPT方法相比较，从而观察该方法在现场监测中的应用价值。方法 按试剂盒说明书操作。胶体染料试条法（DDIA）的原理是胶体金免疫层析法。结果 对552例受检人员进行检查，DDIA法测出阳性数102人，阳性率为18．48％；COPT法测出阳性数84人，阳性率为15．22％。DDIA法敏感性为100．0％，特异性为96．15％，阳性预测值为82．35％，阴性预测值为100．0％，准确性为96．74％。结论 研究表明DDIA法操作方法简单，试剂保存长久（2℃～8℃可保存一年），试验过程较短，并具有敏感性高，特异性强等特点，可作为血吸虫病现场检测和大规模普查的首选方法之一。

Objective To establish a simple, convenient, rapid and exact method for diagnosis of schistosomiasis. Methods Through comparison with the golden standard COPT method, the application value of DDIA in spot test was evaluated. The principle of colloidal gold kit was based on immuno- layer - chromatography. Results With DDIA, the positive rate of suspected cases was 18.48% （51/276）, while COPT was 15.22% （42/276）. The relative sensitivity of DDIA was 100 %, relative specificity was 96.15 %, positive predicting rate was 82.35 %, negative predicting rate was 100 %, veracity achieved 96.74%. Conclusions These results suggest that DDIA is a simple, rapid, highly sensitive and specific method in diagnosis of schistosomiasis; and the test kits reagents can be conserved for a longer time （one year at the temperature ranged from 2℃ to 8℃ ）. So DDIA can be the initial method for the spot test and large scope screening inspection in the epidemic areas.