β_2-微球蛋白试剂对尿素测定结果的影响

Interfering Effect of β_2-microglobulin Reagent on Urea Assay Results

目的研究对尿素自动分析产生污染的项目及解决方法。方法将筛查出对尿素测定有明显影响的β2-微球蛋白试剂加入混合血清,测定其尿素,并与加入生理盐水的对照管进行比较,重复10次,观察分析β2-微球蛋白试剂对尿素测定结果的影响程度。结果β2-微球蛋白试剂对尿素对尿素测定结果产生明显的正干扰。结论自动生化分析中应注意测试项之间试剂对检测结果的影响,可用带模块的生化分析仪排除干扰,或者利用仪器的清洁程序,消除干扰。

Objective To investigate which regent that interfering on the urea auto- assay results, and to find the resolve measures of this effect. Methods β2- microglobulin reagent which was be screened to have apparent interfering effect on the urea assay results was be added into mixed serum. Urea concentration was detected for 10 times within the mixture and the normal control which was added with normal saline, and the effect of β2 - microglobulin reagent to urea results was assessed. Conclusion The influencing effect of β2 - microglobulin reagent on the urea assay results is significant and must be considered in auto clinical chemistry assay. It can be eliminated by clinical chemistry analyzer with modular or the clean procedure of the instrument.