电刺激对海马表达Nogo-A蛋白的影响

Effects of electric stimulation on Nogo-A expression in hippocampus of rats

目的探讨慢性电刺激对大鼠海马表达Nogo-A蛋白表达的影响,以了解海马Nogo-A蛋白升高与癫痫发生的因果关系。方法70只大鼠随机分为5组,即4个电刺激组,1个假电刺激组,分别电刺激1,3,6,9 d,对海马进行免疫组织化学染色和蛋白印迹实验,检测海马表达Nogo-A蛋白的情况。结果假电刺激组14只大鼠行为均无异常；电刺激1d与3d组大鼠亦未发生抽搐；电刺激6d组有3只发生抽搐,电刺激9d组在刺激6d时,有1只发生抽搐,刺激9d时有13只发生抽搐。同时,免疫组织化学实验发现,在电刺激3,6,9d后,海马Nogo-A蛋白表达量与假电刺激组相比均有显著升高(P＜0．05或P＜0．01),其升高趋势呈电刺激时间的依赖性(r=0．775),蛋白印迹实验结果基本相同。结论海马Nogo-A蛋白表达的量与电刺激的时间呈正相关,而在发生癫痫之前大鼠海马Nogo-A蛋白表达已经升高。

Objective To explore the effects of chronic electrical stimulation （ES） on Nogo-A expression in the hippocampus of rats. Methods Thirty male Wistar rats were randomly divided into a control group and 4 experimental groups. The rats in the control group were given sham ES, while those in the experimental groups received 1 , 3, 6, or 9 days of ES before being sacrificed for the detection of Nogo-A expresson in the hippocampus by immunohis- tochemistry and Western blotting. Results There was a positive correlation between the level of Nogo-A expression and the duration of ES, as shown by the immunohistochemistry technique. Western blotting showed the same result. Conclusion In general, seizure occurred 8 days after electrical stimulation began, Elevated Nogo-A expression in the hippocampus began earlier than seizures in the epilepsy model groups.