亚甲蓝与多聚阴离子化合物结合反应的研究

Study on the interaction between methylene blue and compound of mickle anions

对光谱探针亚甲蓝(MB)分别与硫酸化茯苓多糖(SP)、硫酸软骨素(CS)、透明质酸(HA)等多聚阴离子化合物相互作用的吸收光谱进行了比较研究,探讨了CS等多聚阴离子化合物与MB相互作用机理及其与MB结合的功能基团,计算了MB与SP、CS最大结合数分别为54和73。研究结果表明MB与多聚阴离子化合物的磺酸基发生明显的结合反应,与羧基不能发生明显的结合反应,多聚阴离子化合物与MB结合的功能基团是磺酸基。

The absorption spectras of the interaction between the spectroscopic probes methylene blue （MB） and compund of mickle anions （ sulfation pachymaran （SP） , chondroitin sulfate （ CS）, hyaluronic acid （HA） , etc） were copmaratively studied by spectrometry. The mechanism of the interaction and function group between the compound of mickle anions and the methylene blue was also discussed. And the maximum binding numbers of MB - SP and MB - CS were obtained, which were 54 and 73. The results showed that MB could only interact with sulfate group of the compound of mickle anions, while MB could not interact with carboxylic group of compound of mickle anions.