牛和羊胎盘肽的制备及其生物活性的研究

Preparation of bovine and caprine placental peptide and studies on its biological activity

目的探索一种新型的免疫调节剂,充分利用动物胎盘。方法利用超滤法从牛和羊胎盘中提取胎盘肽,采用改良的加脲Tricine-SDS-PAGE法测定其相对分子质量;利用紫外光扫描其最大吸收峰;并通过建立体外抑制兔淋巴细胞模型,利用四甲基偶氮唑盐(MTT)比色法测定所提取胎盘肽的免疫调节作用。结果从牛和羊胎盘中成功提取出了胎盘肽,其相对分子质量分别为4 785和4 386;其最大吸收峰分别为210.5和225.4 nm;牛和羊胎盘肽均可使顺铂抑制的兔外周血T淋巴细胞的转化率显著提高,其中牛胎盘肽以1∶1×103、1∶1×1042组吸收度明显高于抑制组(P<0.05);羊胎盘肽以1∶1×102、1∶1×103和1∶1×1043组效果最为明显(P<0.01)。结论牛和羊胎盘中含有的胎盘肽可成功提取出来,其可明显提高淋巴细胞的转化率,是一种较为理想的免疫调节剂。

Purpose To search for a new immunoregulator and to utilize animal placenta adequately. Methods The distilled bovine and caprine placental peptides were prepared by ultrafiltering and their relative molecular weights were mensurated by improved Tricine-SDS-PAGE eletrophoresis with urea. Their absorbance peaks were got by ultraviolet scanning. Their immnoregulatory effects were mensurated by MTT colorimetric assay after establishing a model of in vitro immunosuppressive lymphocytes of rabbit. Results The distilled bovine and caprine placental peptides were prepared successfully. Their relative molecular weights were 4 785 and 4 386 respectively. And their absorbance peaks were 210.5 nm and 225.4 nm respectively. The bovine and caprine placental peptides could improve immunosuppressive T lymphocyte transformation rate. The immunosuppressive lymphocyte transformation rates in groups with the bovine placental peptide of 1 ： 1 × 10^3 and 1 ： 1 × 10^4 （ P 〈 0.05）and with the caprine placental peptides of 1 ： 1 × 10^2, 1 ： 1× 10^3 and 1 ： 1×10^4（ P 〈 0.01 ）increased more remarkably than that in immunosuppression group. Conclusion There were placental peptides in bovine and caprine placenta which could be distilled successfully. And they could improve immunosuppressive T lymphocyte transformation rate remarkably, and may be a reasonable immunoregulator.