番茄红素预防大鼠高血糖及其作用机制

THE PREVENTIVE EFFECT OF LYCOPENE ON HYPERGLYCEMIA IN ALLOXAN-INDUCED DIABETIC RATS AND ITS MECHANISM

目的:研究番茄红素(lycopene,LP)对大鼠高血糖的预防作用并探讨相关机制。方法:40只SD大鼠随机分为5组,其中3个剂量组分别以番茄红素5、20和50mg/(kgbwd)灌胃,四氧嘧啶模型组和正常对照组以等量色拉油灌胃,连续15d后,模型组和各剂量组大鼠腹腔注射四氧嘧啶(ALx)150mg/kgbw。注射后D4、D14进行口服葡萄糖耐量试验,称体重。D14同时测定糖化血清蛋白(GSB)、血清胰岛素(FISN)、血清游离脂肪酸(NFFA)、肝组织SOD,GSH-Px及MDA等。结果:LP中剂量组大鼠餐后2h血糖、GSP含量显著低于模型组(P<0.01),体重高于模型组(P<0.01);LP各剂量组大鼠肝组织SOD、GSH-Px含量均明显高于模型组,MDA含量低于模型组(P<0.01)。但LP各剂量组FINS和NEFA浓度与模型组比均无显著性差异。结论:预防性给予LP可缓解ALx所致的高血糖,并缓解高血糖大鼠体重下降程度。其机制可能与番茄红素增强机体的抗氧化能力,抑制脂质过氧化等效应有关。

Objective： To study the preventive effect of lycopene （LP） on hyperglycemia in alloxan-induced diabetic rats, and reveal its possible mechanism. Method- Forty Sprague-Dawley rats were randomized into 5 groups： three groups of LP at doses of 5, 20, 50 mg/（kg bw ·d）for 15d, one control group and one model group of salad oil. Then alloxan （ALx） were injected intraperitoneally to the rats at dose of 150 mg/kg bw. At D4 and D14 after ALx injection, an oral glucose tolerance test （OGTT） was performed and body weight was measured, and at D14 GSP, FINS, NEFA, SOD,GSH-Px and MDA in liver were also determined . Results： Compared with the indices in group with ALX injection only, both the 2h blood glucose and GSP in group with medium-dose LP decreased significantly （P〈0.01） , and body weight was remarkably higher（P〈0.01）. In groups with supplement of different doses of LP, SOD, GSH-Px in liver increased significantly and MDA in liver decreased significantly （P〈0.01） . There were no differences in FINS, NEFA among these groups. Conclusion： Oral administration of LP can improve hyperglycemia and body weight loss in ALX-induced diabetic rats. The possible mechanism may be related with the effect of lycopene in increasing antioxidation ability and reducing lipid peroxidation in rats.