染料木黄酮对胃癌细胞侵袭转移的抑制作用

INHIBITION OF GASTRIC CANCER CELLS INVASION AND METASTASIS BY GENISTEIN IN VITRO

目的:研究染料木黄酮(genistein)对胃癌细胞侵袭转移的影响,并初步探讨其作用机制。方法:运用MTT法观察染料木黄酮对人胃癌细胞系SGC-7901细胞粘附能力的影响;用BoydenChamber膜侵袭系统,观察对SGC-7901游走和侵袭的影响;免疫细胞化学法观察对SGC-7901细胞TGF-β1表达的影响。结果:染料木黄酮作用后,SGC-7901细胞粘贴率降低(P<0.05),游走穿膜细胞数(83±4)和侵袭穿膜细胞数(35±3)均明显低于对照组(114±7,60±4,P<0.05),并且能降低TGF-β1的表达。结论:染料木黄酮能有效抑制胃癌侵袭转移。其作用机制可能与通过降低TGF-β1的表达,抑制胃癌细胞异质粘附、运动能力有关。

Objetive： To study the effect of genistein on the invasion and metastasis of human gastric cancer cells SGC-7901 in vitro. Method： MTT was used to study the effects of adhesion by genistein on SGC-7901. The Boyden Chamber membrane invasion culture system was used to study invasion and metastasis. Cell-immunochemistry was used to observe the change of TGF-β1. Results： In the membrane invasion culture system, the numbers of invading and migrating SGC-7901 cells were significantly lower in genistein treated groups than those in the control groups （P〈0.05）. The adhesion rate and TGF-β1 expression were down-regulated in genistein groups （P〈0.05）. Conclusion： Genistein can inhibit the migration and invasion of SGC-7901 gastric cancer cells in vitro, probably via down-regulation of TGF-β1.