用于中草药基因工程的双价双-T表达载体的构建

Construction of A Double T-DNA Plant Expression Vector Containing Two Genes Used in Genetic Engineering of Chinese Medicinal Herbs

以紫外线和水杨酸诱导后的烟草为材料,对经过修饰的烟草I类几丁质酶和I类β-1,3-葡聚糖酶cDNA进行了克隆和序列分析,然后分别加上Hsr203J启动子和NOS终止子,依次插入到同一个双T植物表达载体130上,获得植物双价双T表达载体。经酶切和PCR鉴定证明构建的载体与预期设计的一致。

The modified I-chitinase and β-1,3-Glucanase from the Nicotiana tabacumcv. NC89 was cloned and sequenced after inducing with salicylic acid and ultraviolet radiation, then, ligated them with Hsr203J promoter and NOS terminator respectively. When we cloned them into 130, a plant bivalent expression vector was obtained. The results of the restriction enzymes digestion and PCR demonstrated the correctness of construction.