依贝沙坦对糖基化终产物诱导的肾系膜细胞增殖的影响

Effect of Irbesartan on proliferation of human renal mesangial cell under AGEs

目的观察依贝沙坦对糖基化终产物（AGEs）干预的人肾小球系膜细胞（HRMC）增殖的影响。方法用4-甲基偶氮四唑蓝（methyl thiazolyl tetrazolium，MTT）法观察AGEs对HRMC的增殖作用，以及不同作用时间和不同浓度依贝沙坦对这种增殖作用的影响，并通过流式细胞仪检测细胞周期变化。结果依贝沙坦在一定范围内以时问依赖和剂量依赖关系抑制糖基化终产物诱导的肾小球系膜细胞过度增生，并使G0～G1期细胞比例增高。S期细胞数减少。结论依贝沙坦能够抑制AGEs诱导的HRMC增生。这种作用可能是通过抑制细胞分裂、复制实现的。

Objective To investingate the effect of Irbesartan on proliferation of human renal mesangial cell （HRMC） treated with AGEs. Methods The proliferation of HRMC were induced by AGEs in vitro, and the effects of Irbesartan on the proliferation were assessed by MTT assay. Cell cycle were detected by flow cytometry. Results Irbesartan decreased the proliferation of HRMC under AGEs, and the effects of Irbesartan were time- and dose-dependent. We also observed the decreased S% ,the increased（G0 ～ G1 ）%. Conclusions Irbesartan can inhibit cell division and replication.