侵袭性牙周炎病原微生物的检测

Prevalence of putative periodontal microorganisms in Chinese patients with aggressive periodontitis

目的检测侵袭性牙周炎(AgP)患者和牙周健康者龈下菌斑中的7种病原微生物,旨在寻找AgP的主要致病微生物。方法应用以16SrRNA为基础的聚合酶链反应(PCR)技术,检测55例AgP患者和17名健康对照者龈下菌斑中的7种牙周病原微生物:伴放线放线杆菌(Aa),牙龈卟啉单胞菌(Pg),福赛坦氏菌(Tf),牙密螺旋体(Td),直肠弯曲杆菌(Cr),中间普氏菌(Pi),变黑普氏菌(Pn)。结果55例AgP患者中仅有1例检测出Aa,而在健康对照者中未检出该菌。Pg、Tf、Td和Cr在AgP组的检出率分别为81.8%、83.6%、80.0%和81.8%,显著高于健康对照者(17.6%、11.8%、5.9%、29.4%),差异有统计学意义(P<0.01)。结论Pg、Tf、Td和Cr4种微生物在AgP患者中有较高的检出率,提示它们的共同定植可能在AgP中起重要作用。

Objective To evaluate the prevalence of putative periodontal microorganism in Chinese patients with aggressive periodontitis （AgP）. Methods A total of 72 subgingival plaque samples were collected from 55 AgP patients and 17 healthy subjects. Seven putative periodontal microorganisms including Actinobacillus actinomycetemcomitans （ Aa ） , Porphyromonas gingivalis （ Pg ） , Tannerella forsythensis （Tf） , Treponema denticola（ Td） , Campylobacter rectus（ Cr ） , Prevotella intermedia （Pi） and Prevotella nigrescens （Pn） were detected by using 16S rRNA based polymerase chain reaction（PCR）. Results The prevalence of Aa in AgP patients was very low（ 1.8% ）, while Pg, Tf, Td and Cr were more frequently detected in AgP patients（prevalence： 81.8%, 83.6%, 80% and 81.8% ） than in healthy controls（prevalence： 17. 6%, 11.8%, 5.9%, 29.4%, P〈0.01）. Conclusions The prevalence ofPg, Tf, Tdand Crwere very high in aggressive periodontitis patients, and the combination of these bacteria may play an important role in AgP.