抗菌塑料对其表面菌膜形成的抑制作用研究

Depressing Effect on Biofilm Formation by Antimicrobial Plastics

目的研究抗菌塑料的抗菌效果和在菌液中对菌膜形成的抑制作用。方法采用试管稀释法观察抗菌塑料,对临床分离的常见致病菌株的抑菌效果和抗菌谱;活菌平板计数法及扫描电镜判断两种材料表面菌膜的形成。结果抗菌塑料对铜绿假单胞菌、金黄色葡萄球菌、大肠埃希菌等临床常见致病菌具广谱抗菌作用;菌膜形成可分为沉积、黏附、繁殖和菌膜形成4个阶段,抗菌塑料在菌膜形成前就可以显著抑制致病菌生长,从而抑制菌膜形成,扫描电镜结果证实抗菌塑料表面未见菌膜形成。结论抗菌塑料对临床常见致病菌具广谱抗菌、抑菌作用,可抑制菌膜形成,具抗菌作用的医用导管可为防治医用导管相关感染提供了新的途径。

OBJECTIVE To study the antimicrobial activity of antimicrobial plastics and its depressing effect to biofilm on the plastics＇s surface. METHODS Test tube dilution method was adopted to study the antimicrobial activity and spectrum of antimicrobial plastics. Plate live bacterial recording method and scanning electron microscope（SEM） were introduced to study the formation of biofilm. RESULTS Wide inhibiting spectrum to pathogenic microbe such as Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli was confirmed, and the biofilm was formed after four stages, ie deposit, adhesion, propagation and biofilm formation, and propagation of microbe was found to be inhibited greatly in the surface of antimicrobial plastics, so that the formation of biofilm in the surface of antimicrobial plastics was held back, and the observation of SEM also cpnfirmed that no biofilm was formed in the antimicrobial plastics＇s surface. CONCLUSIONS Antimicrobial plastics could inhibit pathogenic microbe remarkably with wide inhibiting spectrum, and it could also depress the formation of biofilm in the surface, which indicated that it is a promising approach to prevent catheter related infections by adopting antimicrobial catheters.