臭氧被服消毒机杀菌效果的影响因素研究

Influence Factors Acted on Germicidal Efficacy of a Bed Unit Ozone Sterilizer

目的研究臭氧被服消毒机消毒效果的影响因素。方法采用载体定量杀菌法,在不同的臭氧浓度、相对湿度、温度、有机物浓度、载体条件下,观察消毒机杀菌效果。结果开机20min,消毒袋内臭氧达到765.21mg/m3,温度恒定,湿度为50%、70%、90%时,消毒机对金黄色葡萄球菌的杀灭率分别为90.96%、95.46%、99.35%;温度、湿度恒定,含0、25%、50%小牛血清的菌悬液,其细菌杀灭率分别是95.56%、68.36%、66.42%;温度和载体对杀菌效果影响不明显。结论臭氧被服消毒机杀菌效果受相对湿度、有机物影响较大,在实际操作中,应充分考虑这些因素,以保证消毒效果。

OBJECTIVE To study the influence factors acted on germicidal efficacy of a bed unit ozone sterilizer. METHODS All the experiments were carried out according to the Technical Standard for Disinfection. RESULTS After working for 20min, the concentration of ozone in disinfection packet attained to 785.21 mg/m^3 , the killing rates of Staphylococcusaureus were 90. 98%, 95.46%or 99.35% under the relative humidity（RH） of 50% ,70% or 90% at 20℃, the killing rates were 95.58% ,68. 36% or 88.42% at 20℃ and RH 70% in the presence of 0%, 25% or 50% organic substances, the temperature and carriers had little influence on killing rate. CONCLUSIONS Considering the strong influences of relative humidity, and organic substance on ozone sterilizer, we should increase the concentration of ozone or improve the conditions of disinfection in actual use to assure the germicidal efficacy.