中国北方两市部分人群IL-2、IL-4基因多态性与乙肝关联性的研究

The association of IL-2 and IL-4 polymorphisms and hepatitis B in population of north China

目的:探讨北方地区人群IL2、IL4基因SNPs与乙肝发生的关联。方法:提取中国北方两市乙肝患者及健康对照外周血基因组DNA,用PCR方法扩增IL2基因包含突变位点330的片段以及IL4基因包含突变位点590的片段,采用RFLP明确健康个体乙肝患者基因型。采用PHASE10软件构建这3个多态性位点的个体单倍体型。以非条件Logitic回归校正混杂因素,并进行多态性与乙肝风险关联性的统计学分析。结果:2个多态性位点在北方两市部分人群中具有多态性。两组人群中330(IL2)多态性位点差异有统计学意义(P<0.05);590(IL4)多态性位点差异无统计学意义(P>0.05)。这两个位点所构建的单倍体型中,含有GT单倍体的个体在两组人群中具有高度差异(P<0.01)。结论:IL2基因330位点的多态性对于乙肝的发生有着显著的易患关联,其杂合、纯合形式分别增加乙肝患病风险1.67倍和3.81倍。具有GT单倍体的个体对于乙肝的发生同样具有易感关联,其增加乙肝患病风险约为1.94倍。

To Investlsate the association between the single nucleotlde polymor-phisms（SNPs） of transporter associated with interieukin2 （IL-2）, Interteukin4（IL-4） and the susceptibility of hepatitis B in north China. Methods：Genomic DNA was extracted from the peripheral blood leukocytes of hepatitis B patients and healthy controls. Two fragments covering -330 of the IL-2 gene and -590 of the IL-4 gene were amplified by polymerase chain reaotton （POR）. The SNPs were revealed by restriction fragment length polymorphism （FELP）. Software R4ASE 1.0 was used to construct the haplotype of every Individual. Unconditional Logitic resression model was used to analyze the statlstical association of genotyloe or haplotype in two groups adjusted by gender and abe. Results ： There was significant difference in the SNPs of IL2 between the healthy controls and the heptitis B patients in north China. Sisnificant difference was also found in the combination of --/GT to the two Stoups. Conclusion： SNPS of IL2（-330） may have relation to the susceptibility o heptltis B. -/GT was also related to the susceptibility of heptitis B. These findings suggest that SNPs of IL2 is one of the important host factors to the infection of the heptitis B.