摘要
目的:探讨槐定碱(sophoridine,SR)对大鼠大脑皮层、海马谷氨酸(Glu)和γ-氨基丁酸(GABA)免疫阳性神经细胞的影响及中枢作用机制。方法:采用免疫组织化学方法(SABC法)和显微图像分析技术检测SR对大鼠皮层、海马谷氨酸和γ-氨基丁酸免疫阳性神经细胞变化的影响。结果:侧脑室注射槐定碱0.2mg/只,大鼠皮层、海马Glu免疫阳性神经细胞数明显增加,GABA免疫反应阳性细胞数显著下降。结论:SR引起中枢神经系统Glu及GABA的平衡失调可能是导致中枢兴奋作用机制之一。
Objective： To study the effects of sophoridine on the positive cells Glu and GABA immunoreactions in cortex and hippocampus of rats and the mechanism of its central pharmacological effects. Method： Immunohistochemistry method and micrographic analysis technique were employed to monitor the effect of SR on the alterations of positive ceils Glu and GABA immunoreactions n cortex and hippocampus of rats. Result： SR administrated icv 0.2mg/rat surprisingly increased the number of positive ceils Glu immunoreactions but decreased the number of positive cells GABA immunoreactions. Conclusion： The expression imbalance of Glu and GABA in CNS caused by SR may be one of the mechanisms in which LSR leads the effects of excitation in CNS.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2006年第2期17-19,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
宁夏回族自治区自然科学基金项目(C103)
关键词
槐定碱
谷氨酸
Γ-氨基丁酸
sophoridine
glutamate（Glu）
gammaaminobutyric acid（GABA）
CNS
