柠檬酸对CHO细胞生长和代谢的影响

Influence of Citrate on Growth and Metabolism of CHO Cells

研究了重组中国仓鼠卵巢细胞(CHO)批培养过程中柠檬酸对细胞生长和代谢的影响。结果表明:柠檬酸明显抑制了细胞的生长。与对照组相比,添加12 mm o l/L柠檬酸的处理组细胞的葡萄糖比消耗速率(QG lc)降低了37.5%,渗透压提高了10.0%,乳酸生成量与葡萄糖消耗量的比值增加了27.0%,氨生成量与谷氨酰胺消耗量的比值也增加了。在谷氨酰胺代谢过程中,更多的谷氨酰胺经谷草转氨酶途径生成α-酮戊二酸,参与能量代谢。柠檬酸促使细胞更多地被捕获在G 1期,阻碍细胞的DNA合成,抑制细胞增殖,并促进蛋白的表达。

The influences of citrate on cell growth and metabolism were investigated in batch cultures of CHO cells. With the addition of citrate, cell growth is inhibited obviously, while the metabolism is changed. In batch cultures with 12 mmol/L citrate, the specific glucose consumption rate of CHO cells is reduced by 37.5% ,and the osmolarity increases by 10. 0%, the ratios of lactate accumulation to glucose consumption and ammonia accumulation to glutamine consumption increase, compared to those in the medium without citrate. More glutamine was used for energy metabolism through aspartate aminotransferase pathway. CHO cells could be captured in G1 phase by citrate, which could block DNA synthesis, inhibit cell growth and induce protein expression.