新鲜同种异体骨软骨移植修复软骨缺损

Experimental Study on Repair of Articular Cartilage Defects with Growth Factor and Fresh Allogeneic Osteochondral

目的联合应用新鲜同种异体骨软骨移植,和局部注射碱性成纤维细胞生长因子(basic fibroblast growthfactor,bFGF),探讨能否促进关节软骨缺损区新生软骨的形成,提高软骨缺损修复的成功率。方法48只青紫兰兔,96个实验关节,随机分为A、B、C、D组。无菌条件下制作骨软骨缺损模型。在A组缺损区单纯植入新鲜的同种异体骨软骨,B组单纯局部注射重组人bFGF,C组局部注射bFGF后同时植入新鲜的同种异体骨软骨,D组用作空白对照。术后第4、8、12周作大体观察、X线摄片、组织学检查及免疫组化检查。结果移植加注射bFGF组促进软骨缺损修复的效果均好于其他组,图像分析仪进行软骨细胞记数有显著差异(P<0.05),有统计学意义。修复软骨型胶原免疫组化染色强阳性。结论采用新鲜的同种异体骨软骨移植及联合应用碱性成纤维细胞生长因子,二者能起交互作用,促进了新生软骨的形成。

Objective To investigate if fresh allogeneic osteochondral transplantation, combined with basic fibroblast growth factor, could accelerate the formation of new articular cartilage and improve the success in repair of articular cartilage defects. Methods 48 rabbits（96 experimental joints） were used in the experiment. The rabbits were randomly divided into 4 groups ： Fresh allogeneic osteochondral transplantation was migrated into articular cartilage defects in the knee of rabbits in group A; Basic fibroblast growth factor was injected into the defects in group B; Fresh allogeneic osteochondral transplantation was combined with basic fibroblast growth factor in group C; Group D was the control group. The models of the articular cartilage defects were made in asepsis condition. General observation, X-ray examination, histology examination and immunohistochemistry examination were performed at the 4^th week,the 8^th week and the 12^th week after operation. Results The repair effect of articular cartilage defects in group C was better than that in other groups. There were significant differences in counting chondrocytes between group C and other groups （P〈0.05）. Conclusion Fresh allogeneic osteochondral transplantation,combined with basic fibroblast growth factor,could accelerate the formation of new articular cartilage in the repair of articular cartilage defects, and synergistic effects was also obtained.