卵丘细胞对活体取卵来源的裸卵在体外成熟中的作用

Influence of Cumulus Cells on In Vitro Maturation of Nude Oocytes Derived from Ovum Pick Up

本文探讨了卵丘细胞对活体取卵(ovumpickup,OPU)来源的裸卵在体外成熟中的作用。实验分3组:自然裸卵组(NO),裸卵独自成熟培养;共成熟组(CM),自然裸卵与A级卵丘卵母细胞复合体(Cumulus oocyte complexes,COCs)共成熟培养;非裸卵组(NN),正常AB级COCs成熟后,去除卵丘再进行体外受精与体外培养。结果显示:NN组的成熟率最高,其卵裂率及囊胚率高于NO组,但与CM组相似;CM组的成熟率与NO组没有显著差异,但卵裂率和囊胚率均比NO组高。表明OPU来源的裸卵和A级COCs共成熟能显著改善裸卵的发育能力;卵丘细胞对卵母细胞的促成熟作用可直接作用于裸卵,促进裸卵的成熟及随后的发育。

A study on the influence of cumulus cells on in vitro maturation of nude oocytes derived from ovum pick up （OPU）. There were three groups： NO group （in vitro maturation of nude oocytes with uniform cytoplasmic appearance）, CM group （co-maturation of nude oocytes with grade A COCs）, NN group （in vitro fertilization of normal grade A/B oocytes denuded of cumulus cells after maturation, then in vitro cuhure）. The maturation rate of NN group was the highest. Cleavage and blastoeyst rates of NN group were higher than those of NO group, but were similar to those of CM group. CM group did not differ from NO group on maturation rate but was higher in terms of cleavage and blastocyst rate. These results show that the co-maturation of nude oocytes from OPU with grade A COCs can improve the development of nude oocytes; cuntulus cells can facilitate maturation and development of nude oocytes directly.