酶解魔芋飞粉制备高F值寡肽最佳工艺条件的研究

Processing Technology of Oligo-peptides of High Fischer Ratio from Konjac Fly Powder with Protease

目的:为了得到高F值寡肽,需要对蛋白质进行水解。方法:采用酶法水解魔芋飞粉制备高F值寡肽。通过对蛋白质水解度的测定,确定了碱性蛋白酶和链霉蛋白酶的最佳酶解条件:碱性蛋白酶加酶量0.1%,底物浓度3.75%,pH9.0,水解温度45℃,时间5h;链霉蛋白酶加酶量0.03%,pH8.0,水解温度50℃,时间7h。酶解液再经过凝胶层析分离纯化后可用于制备高F值寡肽。

In order to get oligo - peptides of high fischer ratio, The protein should be hydrolyzed. In this paper ,we get oligo - peptides of high fis- cher ratio from konjac fly powder which was hydrolyzed with protease. The optimum conditions of alkaline protease and pronase were established from the degree of hydrolysis： the quantity of alkaline protease is 0.1%, the density of substratum is 3.75 %, pH is 9.0, the hydrolysis temperature is 4593 and the reactive time is 5 hours; the quantity of pronase is 0.03%, pH is 8.0, the hydrolysis temperatuer is 5093 and the reactive time is 7 hours. The hydrolysis fluid would get oligo - peptides of high fischer ratio from separated by gelatin.