摘要
酿酒酵母(Saccharomyces cerevisiae)的生长过程有大量的胞内NADH产生。有氧途径中,胞外的NADH脱氢酶、三磷酸甘油穿梭酶系是线粒体内NADH氧化的最主要机制。该文主要讨论以下三个方面的内容:不同生理环境下促成线粒体胞内NADH氧化的各主要机制的作用;借助电子传递链开启NADH从胞质脱氢酶到线粒体的通道,各代谢动力学的有序进行;各种酶形成超分子复合物,尤其是起关键调控作用的酶形成具相似生理功能的高整合性功能酶。
During growth of Saccharomyces cerevisiae, an excess of NADH is generated in the cytosol. Aerobically, it has been shown that the external NADH dehydrogenase, as well as the glycerol - 3 - phosphate dehydrogenase shuttle are the most important mechanisms for mitochondrial oxidation of cytosolic NADH. This review summarized the recent results showing (i) the contribution of each of the mechanisms involved in mitochondrial oxidation of the cytosolic NADH, under different physiological situations ; (ii) the kinetic and structural properties of these metabolic pathways in order to charmel NADH from cytosolic dehydrogenases to the inner mitochondrial membrane and (iii) the organization in supra- molecular complexes and, the peculiar ensuing kinetic regulation of some of the erzymes leading to a highly integrated functioning of enzymes having a similar physiological function.
出处
《生物技术》
CAS
CSCD
2006年第3期90-93,共4页
Biotechnology