三七药材皂苷类成分的电喷雾离子阱质谱特征图谱研究

ESI-MS behaviors and the fingerprints of saponins in Panax notoginseng burk

应用电喷雾-离子阱质谱技术研究了22种三七皂苷类成分的电喷雾离子化规律和二级质谱碎裂规律.根据三七皂苷类物质的电喷雾质谱行为特征,对三七对照药材皂苷提取物电喷雾质谱一级全扫描图中的主要皂苷成分进行了初步定性分析.同时利用选择性离子检测质谱技术,初步建立了三七对照药材皂苷提取物的特征图谱.该图谱重现性与特征性较好,且简单易于解析,可以用于相关制剂中三七药材的快速指纹鉴别.

The MS behavior of 22 saponins existed in P. notoginseng burk were studied by direct infusion into electrospray ion trap mass spectrometry （ESI - MS） .All saponins have sodium adducts （ [M ＋ Na]^＋ ） （with at least 7 potassium adducts existed） in positive mode and [ M - H ]^- in negative mode. The MS2 fragment of the 22 saponins were the fragments lossing different sugar - group from their parent compounds, the fragmentation information of molecular ions are more abundant in negative mode compared to that of base peak ions in positive mode. The typical fingerprint of P. notoginseng extracts were obtained with positive/negative full - scan and SIM technology. The saponins presented in ESI- MS and SIM - MS spectra can been identify preliminary based on their corresponding ESI- MS behaviors. SIM technology can provide a more simple and intuitionistic fingerprint of P. notoginseng extracts than that of full - scan technology, and very useful for the fingerprint identification of P. notoginseng and related Chin Tradit Pat Med.