摘要
将来源于枯草芽孢杆菌的纳豆激酶(nattokinase,NK)基因转化到人类可以直接食用的,又非常喜爱的蔬菜—番茄中,得到在转录水平表达的转基因植株.通过根癌农杆菌EHA105介导,转化番茄的下胚轴,诱导愈伤组织形成及分化成幼苗,进而再生出完整植株.提取再生植株基因组DNA,通过PCR扩增检测,结果表明纳豆激酶基因已经成功整合到番茄植物基因组中.RT-PCR实验结果证明了纳豆激酶基因在转录水平上有表达.
Nattokinase （NK） is a fibrinolytic enzyme that is extracted from natto. In fact it has enzymatic property of protease . So nattokinase can be developed as a new frbfinolytic medicine. In this research , tomato plants will be transformed to express nattokinase gene. Hypocotyle were cut from ＇ xifen 3＇ （ Lycopersicon esculentum ）, inoculated with Agrobacterium tumefaciens EHA105 containing nk gene. Plants were regenerated on selective media with Kanamysin. The presence of the nk gene in tomato＇s genome was confirmed by PCR in plants surviving sections. RT-PCR results showed that nk gene have bene expressed at the tmnscripition level. And this result haven＇t bene reported in China so far.
出处
《湖北大学学报（自然科学版）》
CAS
北大核心
2006年第2期181-182,186,共3页
Journal of Hubei University：Natural Science
基金
湖北省自然科学基金(No.2003ABA116)
湖北省中药生物技术重点实验室基金资助
关键词
纳豆激酶基因
番茄
遗传转化
根癌农杆菌
nattokinase gene
tomato
genetic transformation
Agrobacterium tumefaciens
