一种新型脱氧核糖核酸序列表征子及其应用于E.coli启动子对RNA转录启动强度的QSAM研究

A Novel DNA Sequence Expresser and its Application in the QSAM Study for Promoter Strength of E.coli Promoter

从天然碱基的36种性质参数出发,通过主成分分析(PCA)技术处理得到了1个显著的主成分得分,并将该得分作为单个碱基的信息描述子———VBPV。进而使用VBPV对38个大肠杆菌(E.coli)启动子序列一级结构进行表征,并结合多元统计方法将表征参数与转录启动强度(PS)成功地建立了定量序列活性模型(QSAM),该模型拟合复相关系数Rcum与交叉检验复相关系数Qcum分别为0.97和0.95。

Via a principle component analysis （PCA） disposal of 36 kinks of property parameters for natural bases, one principle component score is obtained and serves as information descriptor for single base, termed as principle component scores vector of base propertied variables （VBPV）. Primary structure representation for 38 E. coil promoter sequences by VBPV descriptors is combined with multiple statistic methods and a quantitative sequence-activity model （QSAM） is given out between the resulting representation parameter and transcription promoter strength. This model is successful with its Rcum and Qcum respectively of 0.97 and 0.95.