摘要
在滇池水域中取藻样，过滤、接种在MDM培养基中，置于光照温室中培养，提取出叶绿素-a，然后测定在不同波长下的吸光度值，用叶绿素-a法计算公式计算出叶绿素-a含量．确定适宜的波长范围是用叶绿素-a法测定滇池叶绿素-a生长变化的关键．实验研究结果表明，滇池藻类的叶绿素-a吸收光谱有两个最强的收区：一个是在波长为400～460nm的蓝紫光部分，另一个是在波长为640～660nm的红光部分．在这两个波段下，可测定Fe^2＋对滇池叶绿素-a生长的影响情况。
The water samples are taken from Dianchi Lake. After filtered, it is inoculated in MDM, then it is put in glasshouse, the chlorophyll-a of algae is abstracted, the absorbance values of chlorophyll a are measured at the various wavelength, the content of chlorophyll a could be calculated with a formula. Therefore, to determine the suitable scope of wavelength in chlorophyll-a method was a key factor. The experimental results show that absorbance values are strong in the wavelength scope of 400 ～ 460 nm and 640 ～ 660 nm. The case study is carried out by adding Fe^2＋ into experiment sample for measurement effects of Fe^2＋ on ehlorophyll-a in Dianchi Lake under these two wavelength scope.
出处
《昆明理工大学学报（理工版）》
2006年第3期61-63,共3页
Journal of Kunming University of Science and Technology(Natural Science Edition)
基金
国家重点基础研究发展计划项目(项目编号:2002CB412310)
关键词
叶绿素-A
波长
吸光度
藻类
滇池
Chlorophyll -a
wavelength
absorbance
algae
Dianchi
