丁酸钠部分依赖CREB上调急性白血病细胞CD86分子

Sodium butyrate up-regulate CD86 molecule on acute leukemia cells partially dependent CREB

目的:观察丁酸钠(SB)对急性白血病细胞CD86分子表达的影响,并探讨其作用机制。方法:流式细胞术检测NB4、HL-60、U937细胞经SB处理前后的CD86分子表达变化,半定量RT-PCR检测SB对各组细胞CD86 mRNA表达变化,AUT凝胶电泳检测核心组蛋白乙酰化程度,pCREB试剂盒检测细胞核内pCREB含量变化。结果:SB处理各组细胞CD86分子表达与对照组比较均出现显著升高;CD86 mRNA水平表达也明显增高;AUT电泳显示经SB作用后,各组细胞组蛋白乙酰化程度明显增加;SB处理后细胞核内pCREB含量增多。结论:SB使NB4、HL-60、U937细胞核心组蛋白乙酰化程度增加,染色质重塑,有利于CREB等转录因子的活化并与DNA结合,促进CD86转录增强,表达增多。

Objective：To observe the effects of sodium butyrate on the expression of CD86 molecule on acute leukemia cells and explore the mechanisms of action. Methods：The expression of CD86 in NB4, HL-60 and U937 treated by SB or not was assayed by flow cytometric analysis. The alteration of CD86 mRNA was examined by semiquantitative RT-PCR. AUT gel electrophoresis was applied to check the state of histone acetylation. The content of phospho-CREB was assayed by pCREB kit. Results：Up-regulation of CD86 was observed on those cells treated by SB. The levels of CD86 mRNA in SB treated cells were statistically enhanced. The acetylation degrees of SB treated cells were higher than control groups. The contents of phospho-CREB were also raised in SB treated cells. Conclusion：SB can improve the acetylation states of acute leukemia cells, remodel the chromatin which contributes to the binding on DNA of transcription factors ,such as CREB and then promote the transcription and expression of CD86.