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PMA+Ion和PHA用于流式细胞术检测Th1/Th2细胞的结果比较 被引量:5

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出处 《中国免疫学杂志》 CAS CSCD 北大核心 2006年第6期558-559,共2页 Chinese Journal of Immunology
基金 上海市教委青年科学基金资助(04BC30)
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参考文献5

  • 1Shapiro H M. The evolution of cytometen [ J]. Cytometry A ,2004 ;58(1) :13-20.
  • 2Pinto L A, Trivett M T, Wallace D et al. Fixation and cryopreservation of whole blood and isolated mononuclear cells : influence of different procedures on lymphocyte subset analysis by flow cytometry [J].Cytometry B Clin Cytom, 2004;63B(1 ):47-55.
  • 3Godoy-Ramirez K, Franck K, Mahdavifar S et al. Optimum culture conditions for specific and nonspecific activation of whole blood and PBMC for intraceUular cytokine assessment by flow cytometry [ J ]. J lmmunol Methods, 2004;292(1-2) :1-15.
  • 4Rostaing L J, Tkaczuk M, Durand C et al. Kinetics of intracytoplasmic Thl and Th2 cytokinc production assessed by flow cytomctry following in vitro activation of peripheral blood mononuclcar cells [ J].Cytomctry, 1999 ;35:318-328.
  • 5Baran J, Kowalczyk D, Ozog Met al. Three-color flow cytometry detection of intraceUular cytokines in peripheral blood mononuclear cells : comparative analysis of phorbol myristate acetate-ionomycin and phytohemagglutinin stimulation [ J ]. Clin Diagn Lab Immunol, 2001 ;8(2):303-313.

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