PGE_2免疫干预对大鼠Vv攻击后TNF-α、IL-10以及SOD和NO的影响

Effect of immuno-intervention with PGE_2 on TNF-α,IL-10,SOD and NO in rats attacked by vibrio vulnificus

目的:探讨前列腺素E2(Prostagland in E2,PGE2)免疫干预对大鼠创伤弧菌(V ibrio vu ln ificus,Vv)攻击后TNF-α、IL-10以及SOD和NO的影响。方法:正常大鼠和肝病大鼠各27只,分别为Vv攻击对照、Vv攻击后氧氟沙星药物治疗和Vv攻击PGE2氧氟沙星联合保护组(n=9)。结果:无论是正常还是肝病大鼠,PGE2免疫干预组IL-10均比相对应组高,有显著意义(P<0.01),而TNF-α又均比相对应组低(P<0.01);PGE2免疫干预组SOD均较相对应组显著升高,而NO明显降低,有显著意义(P<0.01)。结论:PGE2免疫干预对大鼠Vv攻击后具有免疫保护作用。

Objective： To study of immuno-intervention with prostaglandin E2 （ PGE2 ） on tumor necrsis factor（TNF-α）, interleukin 10（IL-10）, SOD and NO in rats attacked by vibrio vulnificus. Methods：27 normal rats and 27 hepatopathy rats, they were divided into Vv attacked group, ofloxacin treatment group and combined PGE2 with ofloxacin protect group（n = 9）. Results ：The result of cytokines detection showed that serum IL-10 and SOD level of combined PGF2 with ofloxacin protect group was significantly higher than that of ofloxacin treatment group, however, TNF-α and NO level of PGE2 immuno-protect group were significantly lower than those of ofloxacin treatment group（ P 〈 0. 01 ）. Conclusion：This study shows that PGE2 have immuno-protect function to rats attacked by vibrio vulnificus.