RhoA在洛伐他汀抑制HepG_2细胞增殖中的作用

Role of RhoA in Lovastatin-inhibited Proliferation of HepG_2 Cells

目的:探讨RhoA在洛伐他汀抑制HepG2细胞增殖过程中所起的作用。方法:用基因芯片、RT_PCR和Western blot检测洛伐他汀作用前、后HepG2细胞中RhoA的表达。结果:加入洛伐他汀后,mRNA(RhoA)和RhoA蛋白水平均上调;加入香叶基香叶基焦磷酸或法呢基焦磷酸后,RhoA蛋白水平上调被不同程度逆转。结论:减少RhoA香叶酯化,抑制RhoA的细胞膜锚着可能是洛伐他汀抑制HepG2增殖的机制之一。

Objective： To study the role of RhoA in lovastatin-inhibited proliferation of HepG2 cells. Methods： The cDNA microarray assay, RT-PCR and Western blot were carried to analyze the mRNA and protein expression of RhoA, respectively. Results： The mRNA and protein expression levels of RhoA were up-regulated in lovastatin-inhibited HepG2 cells. This response could be reversed by addition of geranylgeranyl pyrophosphate or farnesyl pyrophosphate in different degrees. Conclusion： RhoA isoprenylation inhibition and its deloealization from the cell membrane may be one of the mechanisms of lovastatin-inhibited anti-proliferation effect in HepG2 cells.