青春双歧杆菌细胞壁的免疫调节和体内抑瘤作用研究

STUDY OF IMMUNOMODULATION AND TUMOR-INHI MICROFLORAREGULATION AND INTESTINAL PROTECTION

目的:探讨青春双歧杆菌细胞壁对小鼠腹腔巨噬细胞功能的影响及其对人膀胱癌细胞的体内抑制作用。方法:将青春双歧杆菌细胞壁腹腔注射于KM小鼠,以ELISA法测定腹腔巨噬细胞产生的IL-1、IL-12及TNF-α的含量;建立膀胱癌小鼠模型,腹腔注射双歧杆菌细胞壁,2周后,剥离瘤体,计算抑瘤率。结果:青春双歧杆菌细胞壁注射组腹腔巨噬细胞产生的IL-1、IL-12及TNF-α的含量分别高于对照组,两者比较具有显著差异(p<0.01);细胞壁注射组可以使小鼠局部肿瘤体积减小、肿瘤重量减轻,与对照组之间比较有显著性差异(p<0.01)。结论:青春双歧杆菌细胞壁成分能激活小鼠腹腔巨噬细胞,并且在体内能抑制人膀胱癌细胞的生长。

Objective：To explore the immunologic regulation of the bifidobacterium adolescence cell wall （BCW） on function of macrophage in mice and tumor inhibition of BCW on human transitional cell carcinoma of bladder （ BTCC ） inside of body. Methods： BCW was injected into abdomen of KM mice, the level of IL - 1, IL - 12 and TNF - α which produced by macrophage was determined by ELISA reagent box. BCW was injected in abdomen after establishing human transitional cell carcinoma of bladder, peel off the tumor tissue,weight, calculated the tumor-inhibition rate. Results：The level of IL - 1, IL - 12 and TNF -α of BCW injection group was higher than of control group（p 〈0.01 ） ;After treatment the size and weight was reduced. There was significant different between BCW injected group and control group （ p 〈 0.01 ）. Conclusion： BCW can activate macrophage; BCW can inhibit the growth of BTCC inside of body.