脱落酸处理对铁皮石斛类原球茎体耐脱水性的影响

The Influence of Abscisic Acid Treatment on Desiccation Tolerance in Dendrobium candidum Protocorm-like Bodies

脱落酸(abscisic,ABA)预处理可以显著提高铁皮石斛(Dendrobiumcandidum)类原球茎体(protocrom-likebodies,PLBs)的耐脱水性,其中以5μmol/L的ABA预处理24h效果最好。经2h快速脱水后,类原球茎体存活率约为对照的10倍,达到50%左右。预处理期间细胞相容性物质含量的研究表明,ABA预处理过程中可溶性总糖、蔗糖、可溶性蛋白质及脯氨酸含量均无明显变化,而可溶性多糖含量明显增加,作者认为可溶性多糖的积累是类原球茎体耐脱水性提高的原因之一。

Effect of ABA on desiccation tolerance in the protocorm-like bodies （PLBs） of D.candidum was studied. The best result was obtained while ABA precultured at 5 μmol/L for 24 h （Fig. 2）. PLBs vitality rate was 10 times of the control after 2 h desiccation （Table 1）. Detection of cytoplasmic osmoticums showed that ABA preculture could not induce the accumulations of soluble sugar, proline, etc （Tables 3, 4 and 7）. But the content of soluble polysaccharide rose conspicuously （Table 5）. So we think that the accumulation of soluble polysaccharide may play an important rolein desiccation tolerance induced by ABA preculture.