17-β雌二醇对肾间质纤维化防治作用的探讨

17β-estradiol study of effect of estrogen preventing renal interstitial fibrosis

目的探讨17-β雌二醇对肾间质纤维化防治作用可能的机制。方法雌性SD大鼠30只，随机分为4组：①对照组；②生理雌激素组；③低雌激素组；④17-β雌二醇组。21d后光镜观察单侧梗阻肾组织病理改变；通过免疫组化方法检测转化生长因子（TGF-β1）、α-平滑肌动蛋白（α-SMA）；并用RT-PCR方法检测肾组织α-SMAmRNA的表达。结果单侧输尿管梗阻各组出现肾小管间质纤维化改变，其肾组织TGF-β1。和α-SMA表达上调（P〈0．01）；与生理雌激素组相比，低雌激素组纤维化病变加重，上述物质表达均明显增多（P〈0．05）；而17-β雌二醇组则病变均减轻（P〈0．05）。结论注射17-β雌二醇可能通过下调TGF-β1，和α-SMA的表达，抑制细胞外基质的生成，进而韶到延绣肾间后纤维化的作用．

Objective To evaluate the effect of 17β-esfradiol on renal interstitial fibrosis and to explore the possible mechanism. Method Thirty Sprague-Dawley female rats were randomly divided into four groups, control group; normal estrogen group （ rats only underwent UUO） ; low estrogen group （rats were ovariectomized and underwent UUO）; high estrogen group （rats underwent UUO and received 17β-estradiol injection）. The rats were sacrificed after 21 days, renal tissues were examined by using PAS and Masson stains. Immunohistochemistry was used to determine the protein expressions of TGF-β1 and α-SMA, the mRNA expressions of α-SMA were detected with reverse transcription-polymerase chain reaction. Results Compared with control group, there appeared tubulointerstitial fibrosis developed in UUO groups, the expression of TGF-β1 and α-SMA increased remarkably （P〈0. 01）. Compared with UUO group, the lesion was aggravated significantly in UUO ＋ OVX group, with enhanced expression of TGF-β1 andαSMA enhanced （P〈0. 05）, and in UUO ＋ E2 group the lesion was attenuated,and the expression of those parameters decreased （P〈0. 05）. Conclusions 17β- esfradiol may prevent the development of interstitial fibrosis by inhibiting the over expression of TGF- β1 and αSMA, which may consequently result in the production of extracellular matrix deposition.