一侧肾切除大鼠术后留存肾代偿早期结缔组织生长因子和血管内皮生长因子的表达

The expression of connective tissue growth factor and vascular endothelial growth factor in remnant rat kidney during the early process of compensatory renal growth following unilateral nephrectomy

目的观察一侧肾切除大鼠术后留存肾早期结缔组织生长因子(CTGF)及血管内皮生长因子(VEGF)表达的改变,探讨一侧肾切除大鼠留存肾的代偿及肥大的机制。方法SD大鼠48只,分为2组:⑴实验组24只,行右肾切除;⑵对照组24只,不做任何处理,2组分别于实验第1、4、8、16 d随机处死各6只。留取左肾作病理及CTGF及VEGF免疫组化检查。结果⑴手术组左肾增加明显,至第16天明显重于第1天(P<0.01),与对照组同期相比,增重明显(P<0.05)。⑵手术组第16天与第1天比较VEGF、CTGF表达增加,肾小球面积及肾小球体积增加(均P<0.05),与对照组同期比较,以上指标均增加(P<0.05)。结论一侧肾切除大鼠留存肾肥大,CTGF及VEGF在留存肾代偿早期增加。

Objective To study the role of connective tissue growth factor （CTGF） and vascular endothelial growth factor （VEGF） in the early stage of compensatory renal growth ,and to explore, the mechanisms of renal compensatory hypertrophy on rat kidney following unilateral nephrectomy. Methods The 48 Sprague Dawley rats were randomly divided into 2 groups with 24 rats in each. The rats in the operation group were underwent right nephrectomy operation and those of the control group were without any treatment. Six rats of each group were sacrificed on the I st, 4th,8th and 16th day of the experiment, respectively. The immunohistochemical method was used to investigate the expressions of CTGF and VEGF in the remnant left kidney of rats in each group. Results In the operation group, the weight of left kidney was increased obviously and the left kidney on the 16th day was obviously heavier than that on the 1st day of the experiment （ P 〈 0. 01 ）. In the operation group, the expression of CTGF and VEGF protein, the glomerular area and volume were increased on the 16th day of the experiment compared with those of the I st day （all P 〈 0. 05 ）. Furthermore, the indexes mentioned above in the operation group were much higher than those of the control group in the same time of the experiment （ all P 〈 0. 05 ）. Conclusion The compensatory hypertrophy occurs in the remnant kidney of rats following unilateral nephrectomy. In addition, the expressions of CTGF and VEGF are increased in the early compensatory state of the remnant kidney.