核因子-κB在高脂饮食兔颈总动脉球囊损伤后的动态变化及其与内膜增生的关系

Relationship between intimal hyperplasia and NF-κB expression in carotid artery of hypercholesterolemic rabbits after balloon injury

目的观察高脂饮食兔颈总动脉球囊损伤后核因子κB(NF-κB)表达的变化及其与动脉内膜增生的关系。方法对高脂饮食兔颈总动脉球囊损伤后不同时间段的颈总动脉标本采用组织形态学方法检测内膜增生;采用原位杂交方法检测损伤血管NF-κB mRNA的表达。结果⑴球囊损伤后,内膜面积随时间逐步增生,中层各时段均未见明显变化,内膜/中层比率随时间逐步增长。对照侧内膜轻度增生但明显较损伤侧轻(P<0.05)。⑵球囊损伤后6 h即可见NF-κB表达,并于2周达高峰,4周仍有较强表达。NF-κB在损伤侧表达均强于对照侧(P<0.05)。结论高脂饮食兔颈总动脉球囊损伤后,NF-κB被激活,从而引起炎症反应,导致内膜增生可能是再狭窄发生的重要机制之一。

Objective To investigate the expression of nuclear factor kappa B （NF-kB） in carotid artery of hypercholesterolemic rabbits injured by balloon and its relationship with intimal hyperplasia. Methods Twenty-five New Zealand male rabbits were fed with atherogenic diet and balloon injury was conducted in right carotid artery 4 weeks later. The rabbits were killed at the 6th hour, 24th hour, I th week, 2th week and 4th week, respectively. Blood samples and right carotid artery were collected in time and intimal hyperplasia was studied by histological morphology method. The expression of NF-kB was measured by in-situ hypridization （ISH）. Results Intimal hyperplasia was present at the 7th day after balloon injury in hypercholesterolemic rabbits and it became more obvious with the increasing of the time. The intimal hyperplasia was not detected in the media area. The intima/media ratio was increased as time went on. The expression of NF-kB mRNA was observed in the carotid atery of hypercholesterolemic rabbits at the 6th hour after balloon injury and the expression reached its peak at the 2th week. The expression of NF-kB was much higher in injury group than that of control group. Conclusion The expression of NF-kB is up-regulated in carotid artery of hypercholesterolemic rabbits injured by balloon, which is involved in the inflammatory reaction and restenosis caused by balloon injury.