尼美舒利对人小细胞肺癌细胞株NCL-H446增殖的抑制作用

Inhibitory Effects of Nimesulide on Proliferation of Human Small Cell Lung Cancer Cell Line NCL-H446

目的研究尼美舒利对人小细胞肺癌细胞株NCL-H446增殖的抑制作用,为临床治疗提供实验室依据。方法常规培养人小细胞肺癌细胞株NCL-H446,MTT法检测尼美舒利的细胞毒性作用;倒置显微镜与Hoechst33258荧光染色观察细胞形态学变化;流式细胞术(FCM)分析细胞凋亡率。结果MTT显示尼美舒利呈剂量与时间依赖性抑制人小细胞肺癌细胞株NCL-H446的增殖;倒置显微镜下可见细胞变小、变圆、皱缩、部分细胞脱落飘浮于培养基中。荧光显微镜下可见细胞核染色质致密浓缩、荧光强度增强、染色质边集、核破碎,凋亡小体形成等。FCM检测结果表明尼美舒利既可诱导NCL-H446细胞凋亡,又可引起坏死,400μmol/L的尼美舒利作用细胞24h后,细胞早期凋亡率为7.65%,晚期凋亡率为29.76%,细胞坏死占23.9%。结论尼美舒利对体外培养的人小细胞肺癌细胞株NCL-H446有较显著的抑制作用,该作用与诱导细胞凋亡与坏死有关。

Objective To study the effects of nimesulide on proliferation of human small cell lung cancer （HSCLC） cells NCL-H446 in vitro to provide an experimental basis for clinical application. Methods NCL-H446 cells were cultured with RPMI1640 medium in regular condition. Cytotoxicity of nimesulide was examined by MTT assay. The morphological change was observed by microscope. Apoptosis were detected by flow cytometry. Results Proliferation of NCL-H446 was inhibited significantly by nimesulide in a dosage and time dependant manner. Under inverted microscope and fluorescence microscope, some NCL-H446 cells undergo a typical apoptosis after 24 hours incubation with nimesulide. Flow cytometry indicates nimesulide can induce 29. 76% NCL-H446 cells apoptosis and 23.9% NCL-H446 cells necrosis after 24 hours incubation with 400mol/L nimesulide. Conclusion Nimesulide can inhibit proliferation of NCL-H446 cells, which may be related to the mechanism of inducing cellular apoptosis and necrosis.