摘要
目的研究siRNA对生长抑素(SOM)基因表达的抑制作用。方法根据SOM全长cDNA序列设计和合成RNA干扰的靶序列,应用T7RiboMAXTM体外转录合成siRNA并转染胃癌细胞系BGC-823,经RT-PCR,免疫组化法检测SOMmRNA和蛋白的表达水平。结果siRNA可以特异性抑制SOM基因的表达。结论通过体外合成siRNA可以特异性抑制SOM基因的表达,为筛选抑制SOM基因表达的有效序列研究奠定了基础。
Objective To study the somatostatin （SOM） gene expression inhibition of siRNA. Methods According to the gene sequene of SOM in the Genbank,we designed the targeted siRNA templates. The siRNA was synthesized by T7RiboMAXTM Expression RNAi system in vitro and was transfected into gastric cancer cell line BGC-823. The inhibitory effects were detected by RT-PCR and Immunohistochemi- cal techniques. Results SiRNA inhibited SOM gene expression. Conclusion SiRNA was generated in vitro and it inhibited SOM gene expression specifically. Our research provides basic research for selecting efficient sequence of suppressing SOM gene expression.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2006年第6期414-416,F0003,共4页
Cancer Research on Prevention and Treatment
关键词
RNA干扰
SIRNA
SOM
抑制
RNA interference
Small interfering RNA, Somatostatin
Inhibit
