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βig-h3 mRNA在人肝癌细胞系中的差异表达及其意义

Differential expression of βig-h3 mRNA in human hepatoma cell lines
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摘要 目的:测定转化生长因子β(TGF-β)诱导分子βig-h3 mRNA在人肝癌细胞系中的表达情况。方法:培养人肝癌细胞T7721(转染并稳定高表达HAb18G/CD147)、7721(未转染HAb18G/CD147)、人肝细胞癌细胞系(HHCC)和正常人张氏肝细胞QZG,提取总RNA,设计合成特异性引物,以β-肌动蛋白(β-actin)作为内参照,检测βig-h3 mRNA的差异表达情况。结果:逆转录聚合酶链反应法(RT-PCR)结果显示,TGF-β诱导分子βig-h3的mRNA在人肝癌细胞中的表达水平明显高于正常肝细胞(P<0.01),其在人肝癌细胞系中的表达水平依次为HHCC>T7721>7721,在正常肝细胞QZG中表达水平最低。结论:证明了βig-h3 mRNA在人肝癌细胞系中高表达,为进一步探讨βig-h3在肝癌细胞黏附和转移过程中的作用机制奠定了基础。 Objective: To screen the differential expression of TGFβ-induced molecule βig-h3 mRNA in hepatoma cell lines. Methods: The human hepatoma cells T7721 which was stably transfected with HAb18G/CD147, non-transfected 7721, HHCC and normal human liver cell QZG were cultured previously. The total RNA was extracted and a pair of special primers was synthesized. RT-PCR was employed to investigate the differential expression of βig-h3 mRNA in human hepatoma cell lines and normal liver cells. Results: The results of RT-PCR suggested that the expression of βig-h3 mRNA in human hepatoma cells was higher than that in normal human liver cell QZG(P〈0.01 ). Its expression level in human hepatoma cells in turn was HHCC 〉T7721 〉7721. Conclusion: This study demonstrates that the expression of βig-h3 mRNA in hepatoma cell lines is higher than that in normal liver cell QZG, which provides a sound basis for exploring the function of βig-h3 in processes of adhesion and metastasis of human hepatoma cells.
出处 《医学研究生学报》 CAS 2006年第6期492-494,共3页 Journal of Medical Postgraduates
基金 国家自然科学基金资助项目(批准号:30200144)
关键词 βjg-h3/TGFBI 肝癌 HAB18G/CD147 黏附 βig-h3/TGFB-induced molecule Hepatoma HAb18G/CD147 Adhesion
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参考文献11

  • 1Skonier J,Neubauer M,Madisen L,et al.cDNA cloning and sequence analysis of βig-h3,a novel gene induced in a human adenocarcinoma cell line after treatment with transforming growth factor-beta[J].DNA Cell Biol,1992,11(7):511-522.
  • 2Skonier J,Bennett K,Rothwell V,et al.βig-h3:a transforming growth factor-b-responsive gene encoding a secreted protein that inhibits cell attachment in vitro and suppresses the growth of CHO cells in nude mice[J].DNA Cell Biol,1994,13(6):571-584.
  • 3Park SW,Bae JS,Kim KS,et al.βig-h3 promotes renal proximal tubular epithelial cell adhesion,migration and proliferation through the interaction with alpha3beta1 integrin[J].Exp Mol Med,2004,36(3):211-219.
  • 4成胜权,王文亮,晏伟,李擒龙,王文勇.凋亡抑制基因Survivin在人肝细胞肝癌中的表达及其临床意义[J].医学研究生学报,2004,17(4):296-299. 被引量:6
  • 5Thapa N,Kang KB,Kim IS.βig-h3 mediates osteoblast adhesion and inhibits differentiation[J].Bone,2005,36(2):232-242.
  • 6Bae JS,Lee SH,Kim JE,et al.βig-h3 supports keratinocyte adhesion,migration,and proliferation through alpha3beta1 integrin[J].Biochem Biophys Res Commun,2002,294(5):940-948.
  • 7Kim JE,Kim SJ,Jeong HW,et al.RDG peptides released from βig-h3,a TGF-beta-induced cell-adhesive molecule,mediate apoptosis[J].Oncogene,2003,22(13):2045-2053.
  • 8Genini M,Schwalbe P,Scholl FA,et al.Isolation of genes differentially expressed in human primary myoblasts and embryonal rhabdomyosarcoma[J].Int J Cancer,1996,66(4):571-577.
  • 9Schenker T,Trueb B.Down-regulated proteins of mesenchymal tumor cells[J].Exp Cell Res,1998,239(1):161-168.
  • 10Zhao YL,Piao CQ,Hei TK.Downregulation of βig-h3 gene is causally linked to tumorigenic phenotype in asbesteo treated immortalized human bronchial epithelial cells[J].Oncogene,2002,21(49):7471-7477

二级参考文献18

  • 1[1]Sarela AI,Verbeke CS,Ramsdale J et al.Expression of survivin,a novel inhibitor of apoptosis and cell cycle regulatory protein,in pancreatic adenocarcinoma[J].Br J Cancer,2002,86(6):886-892.
  • 2[2]Mori A,Wada H,Nishimura Y et al.Expression of antiapoptosis gene survivin in human leukemia[J].Int J Hematol,2002,75(2):161-165.
  • 3[3]Das A,Tan WL,Teo J et al.Expression of survivin in primary glioblastomas[J].J Cancer Res Clin Oncol,2002,128(6):302-306.
  • 4[4]Ambrosini G,Adida C,Sirugo G et al.Induction of apoptosis and inhibition of cell proliferation by surviving gene targeting[J].J Biol Chem,1998,273(18):11177-11182.
  • 5[5]Tamm I,Wang Y,Sausville E et al.IAP-family protein survivin inhibits caspase activity and apoptosis induced by Fas (CD95),Bax,caspases,and anticancer drugs[J].Cancer Res,1998,58(23):5315-5120.
  • 6[6]Adida C,Berrebi D,Peuchmaur M et al.Anti-apoptosis gene,survivin,and prognosis of neuroblastoma[J].Lancet,1998,351(9106):882-883.
  • 7[7]Li F,Ambrosini G,Chu EY et al.Control of apoptosis and mitotic spindle checkpoint by survivin[J].Nature,1998,396(6711):580-584.
  • 8[8]Ito T,Shiraki K,Sugimoto K et al.Survivin promotes cell proliferation in human hepatocellular carcinoma[J].Hepatology,2000,31(5):1080-1085.
  • 9[9]Okada E,Murai Y,Matsui K et al.Survivin expression in tumor cell nuclei is predictive of a favorable prognosis in gastric cancer patients[J].Cancer Lett,2001,163(1):109-116.
  • 10[10]Lu CD,Altieri DC,Tanigawa N.Expression of a novel antiapoptosis gene,survivin,correlated with tumor cell apoptosis and p53 accumulation in gastric carcinomas[J].Cancer Res,1998,58(9):1808-1182.

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