βig-h3 mRNA在人肝癌细胞系中的差异表达及其意义

Differential expression of βig-h3 mRNA in human hepatoma cell lines

目的:测定转化生长因子β(TGF-β)诱导分子βig-h3 mRNA在人肝癌细胞系中的表达情况。方法:培养人肝癌细胞T7721(转染并稳定高表达HAb18G/CD147)、7721(未转染HAb18G/CD147)、人肝细胞癌细胞系(HHCC)和正常人张氏肝细胞QZG,提取总RNA,设计合成特异性引物,以β-肌动蛋白(β-actin)作为内参照,检测βig-h3 mRNA的差异表达情况。结果:逆转录聚合酶链反应法(RT-PCR)结果显示,TGF-β诱导分子βig-h3的mRNA在人肝癌细胞中的表达水平明显高于正常肝细胞(P<0.01),其在人肝癌细胞系中的表达水平依次为HHCC>T7721>7721,在正常肝细胞QZG中表达水平最低。结论:证明了βig-h3 mRNA在人肝癌细胞系中高表达,为进一步探讨βig-h3在肝癌细胞黏附和转移过程中的作用机制奠定了基础。

Objective： To screen the differential expression of TGFβ-induced molecule βig-h3 mRNA in hepatoma cell lines. Methods： The human hepatoma cells T7721 which was stably transfected with HAb18G/CD147, non-transfected 7721, HHCC and normal human liver cell QZG were cultured previously. The total RNA was extracted and a pair of special primers was synthesized. RT-PCR was employed to investigate the differential expression of βig-h3 mRNA in human hepatoma cell lines and normal liver cells. Results： The results of RT-PCR suggested that the expression of βig-h3 mRNA in human hepatoma cells was higher than that in normal human liver cell QZG（P〈0.01 ）. Its expression level in human hepatoma cells in turn was HHCC 〉T7721 〉7721. Conclusion： This study demonstrates that the expression of βig-h3 mRNA in hepatoma cell lines is higher than that in normal liver cell QZG, which provides a sound basis for exploring the function of βig-h3 in processes of adhesion and metastasis of human hepatoma cells.