二氧化锆梯度复合羟基磷灰石生物材料的制备及其生物相容性

Preparation and biocompatibility of graded zirconia-hydroxyapatite composite bioceramic

采用干铺-烧结法制备了一种以二氧化锆（ZrO2）为基体梯度复合羟基磷灰石（HAP）的新型生物复合材料，并对其力学性能、微观结构和生物相容性进行了研究。用扫描电镜（SEM）、X射线衍射仪（XRD）、透射电镜（TEM）和EDAX能谱仪对粉体和复合材料进行分析。用复合材料的浸提液进行小鼠急性毒性试验、细胞毒性试验、体外溶血试验以及兔肌肉和骨内材料植入试验，评价复合材料的生物相容性。研究结果表明，复合材料的表面涂层厚度约80μm，芯部基体组织与表面均匀过渡结合，结合良好，没有明显界面，涂层结合强度为15．1MPa，最大弯曲强度为1112．24MPa，KIC为7．3～11．4MPa·m^1/2。其组织相容性好，具有良好的骨传导作用，促进骨组织生长，是一种较理想的新型骨科植入材料。

A new bio-composite material, graded zirconia-hydroxyapatite composite Bioceramic, has been prepared by the dry-laying ＆ sintering method, and its mechanical properties, microstructure and biocompatibility have been studied. The powders and the composite material were investigated using SEM, XRD, TEM and EDAX. To evaluate its biocompatibility, the extract of the composite material was used to carry out a mouse acute toxicity test, a cyto toxicity test, an in vitro hemolytic test, and a rabbit muscle and bone implantation experiment. The results show that the coating of the composite material is about 80μm thick, and is well bonded with the core matrix without obvious interface. The bond strength is 15.1 MPa, the maximum flexural strength is 1112. 24 MPa, and KIC is 7.3- 11.4 MPa·m^1/2. The graded zirconia-hydroxyapatite composite bioceramic has excellent biocompatibilities and bone conductivities, which can promote the growing of bone issues, so it is an ideal bio-composite material for orthopaedic implantation.