超临界CO_2萃取与传统方法提取鸦胆子油实验比较

Comparative Research on Brucea Javanica (L.) Merr. Oil Prepared by Supercritical Fluid Extraction and Traditional Methods

应用超临界CO2萃取法(SCFE)和溶剂法提取鸦胆子油,对两种提取方法制备的鸦胆子油进行了得率、制备工艺、外观评价和化学组成等方面的对比实验,结果表明:SCFE方法萃取的鸦胆子油得率为18.26%,溶剂法的得率为20.25%,两种萃取方法所得鸦胆子油的主要脂肪酸组成为油酸、亚油酸、棕榈酸和硬脂酸。体外试验表明鸦胆子油对肝癌和鼻咽癌有较好的抑制作用。

Brucea Javanica（L.） Merr. Oil was extracted by different methods including supercritical CO2 extraction （SCFE） and organic solvent extraction （SE）. The yield, chemical constituents and flavor character of the oil were studied in comparison. Experimental results show that the yield of Brucea Javanica（L.） Merr oil extracted by SCFE is 18.26 %, lower than that by SE （20.25 %）. There are 4 main fatty acids in Brucea Javanica（L.） Merr. Seed oil made by SCFE and SE, including oleic acid, linolic acid, palmitic acid, stearic acid. The result shows hepatocellular carcinoma and nasopharyngeal carcinoma can be suppressed by Brucea Javanica （L.） Merr. Oil in vitro test.