类多囊卵巢综合征大鼠模型的比较研究

Comparison of Polycystic Ovary-Like Syndrome Models in Rats Established by Subcutaneous Injection of Testosterone Propionate or HCG

目的复制用雄激素及人绒毛膜促性腺激素(HCG)诱导的两种类多囊卵巢综合征(PCOS)大鼠模型,比较两种模型的差异。方法模型1组给9日龄SD雌性大鼠注射丙酸睾丸酮,模型2组给予24日龄SD雌性大鼠皮下埋植左炔诺孕酮,3 d后所有模型2组大鼠皮下注射HCG 9 d。复制出两种类PCOS大鼠模型,分别观察两组模型的排卵情况、卵巢形态学改变、血浆雄激素(T)、雌二醇(E2)、卵泡刺激素(FSH)、促黄体生成素(LH)、空腹血糖(FPG)、空腹胰岛素(FINS)水平。结果模型1组与模型2组大鼠卵巢均成多囊性改变,卵泡囊性扩张,颗粒细胞层数减少、膜间质细胞增生;但模型1组大鼠卵巢体积较其正常对照组缩小,模型2组大鼠卵巢体积较其正常对照组增加。模型1、2组大鼠血清总T、FINS、FPG水平均明显升高;模型1组大鼠血清E2水平较其正常对照组升高,血清FSH、LH水平较其正常对照组降低;模型2组大鼠血清FSH、E2水平较其正常对照组下降,血清LH水平较其正常对照组升高。结论丙酸睾丸酮和HCG诱导的两种类型PCOS大鼠动物模型多囊卵巢、高雄激素血症、高胰岛素血症等现象均与人PCOS类似,两组虽然在某些方面与人PCOS存在一定差异,但却各自有其与人PCOS相似的特征,两者均可作为类PCOS动物模型用于不排卵的研究目的。

Objective Two polycystic-like ovary syndrome models in rats induced by testosterone or HCG were compared. Methods In the model 1, Female Sprague-Dawley rats at age of 9 days were given subcutaneous injection of testosterone propionate. In the model 2, Female Sprague-Dawley rats at age of 24 days were given subcutaneous implant of levonorgestrel. 3 days later HCG was subcutaneously injected to these rats for 9 days. Then the ovulation, morphology of ovary, blood serum levels of testosterone （T）, estradiol （E2）, follicular stimulating hormone （FSH）, luteinzing hormone（LH）, fasting plasma glucose（ FPG）, fasting insulin （FINS） in the two models were assyed. Results Rats of both two models showed polycystic ovary-like syndrome manifestation. But the ovary of rats in model 1 was smaller than that of controls, and bigger in model 2. Serum levels of T, FPG, FINS increased in two models. Serum levels of E2 increased, FSH and LH decreased in model 1. Serum levels of FSH and E2 decreased, LH increased in model 2. Conclusion The two polycystic ovary-like syndrome rat models manifested with PCO, hypertestosteronemia and hyperinsulinemia. Those rats showed similar changes in human PCOS, and can be used as model animals for certain rat experimental research.