人工饲养恒河猴血液生化正常参考值的测定与分析

Measurement and Analysis of Hematological and Blood Biochemical Indices in Bred Rhesus Monkeys

目的了解人工饲养条件下不同地域来源恒河猴血液学和血液生化值之间的差异和相互关系。方法应用全自动血液分析仪和生化分析仪测定恒河猴的血液学指标22项和血液生化指标19项,同时用统计学方法分析比较了相同性别和相同年龄阶段的海南和广西恒河猴的血液学和血液生化值的差异及相互关系。结果在所测定的血液生化值中,海南猴的血糖(GLU)值都明显高于同组的广西猴;成年的海南猴的肌酐(Cr)、白蛋白(ALB)、间接血清胆红素(IBIL)明显低于成年的广西猴。海南恒河猴的红细胞平均比容(MCV)值明显高于同组的广西猴;雌性组中,海南恒河猴的嗜酸细胞总数(EOS)、嗜酸细胞百分数(EOS%)、MCV、单核细胞百分数(MONO%)、红细胞平均血红蛋白量(MCH)明显偏高;青幼年组中,海南恒河猴MCV、中性粒细胞总数(NEU)明显偏低;成年组中,海南恒河猴MCV、NEU明显偏高,而MONO的值明显偏低。结论除了年龄和性别为主要的影响因素外,地域来源的不同也会影响到恒河猴血液学和血液生化参考值的差异。

Objective To investigate the hematological and blood biochemical parameters of rhesus monkeys raised in different geographic regions. Methods Twenty-two hematological and nineteen blood biochemical indices of rhesus monkey bred in Hainan and Guangxi were measured using an automatic blood cell analyzer and automatic blood biochemical analyzer. Statistical comparisons of the results were made between these two breeds of monkeys of the same gender and age. Results The reference range values of Cr, ALB, IBIL were higher in adult Guangxi monkeys compared to those in Hainan monkeys. EOS, EOS%, MCV, MONO% , MCH were higher in female Hainan monkeys compared to those of Guangxi monkeys. MCV and NEU were higher in juvenile Guangxi monkeys than those in Hainan monkeys. MONO was higher, MCV, NEU were lower in adult Guangxi monkeys compared to those in Hainan monkeys. Conclusion Except the age and gender, the conditions in different geographic regions significantly affect blood parameter value in rhesus monkeys.