甲基对硫磷水解酶OPHC2的序列分析及结构预测

Sequence analysis and structure prediction of methyl parathion hydrolase OPHC2

将氨基酸的多序列局部比对、基于结构的序列比对和氨基酸替换忍耐性分析等方法联合起来对甲基对硫磷水解酶OPHC2进行了序列分析,确定出4组保守性功能序列。根据其高级结构发现这类酶具有典型的α/β(TIM)折叠桶结构,由8个折叠结构构成活性中心区域,并且这4组保守序列都位于这8个折叠结构上。通过对这4组保守序列的进一步分析发现当一个序列中同时含有它们时,这个序列为甲基对硫磷水解酶的可能性大于60%。

Four sequence motifs of methyl parathion hydrolase OPHC2 have been found by sequence local alignment, structure - based sequence alignment and the analysis of substitution patterns of residues. It has a representative α/β barrel structure consisting of eight parallel β - strands that formed the active sites connected by α - helices from its predicted structure. The four sequence motifs have been located in the eight parallel β- strands. When the sequence of an enzyme contains all the four motifs, it can be identified as methyl parathion hydrolase and the correct rates of identification are over 60%.