稻瘟菌Ⅰ型烯醇化酶基因全长cDNA的电子克隆(英文)

In silico cloning of MgEno-1 cDNA from Magnaporthe grisea

利用电子克隆技术从稻瘟菌中克隆到一个新的Ⅰ型烯醇化酶全长cDNA,暂命为MgEno-1。MgEno-1全长1571核苷酸,其预测的ORF为1317核苷酸,共编码438个氨基酸。起始密码子ATG位于第53位,终止密码子TAA位于第1369位。序列分析表明该烯醇化酶与丝状真菌中已报道的其它烯醇化酶高度同源,且长度一致,这暗示烯醇化酶基因进化上高度保守,甚至有可能像18SrRNA一样可作为进化尺度。这将是第一个用电子克隆技术从稻瘟菌中克隆到的基因。

Here we report a novel cDNA clone（ MgEno - 1 ） encoding α - enolase in rice blast fungus, Magnaporthe grisea （ M. grisea）, acquired by in silieo cloning strategy.The MgEno - 1 consists of 1571 base pairs（bp）in length, and was predicted to contain a 1317 bp of open reading framework（ORF） corresponding to 438 deduced amino acids（aa）, with ATG initial codon and TAA stopping eodon at the position of 53 and 1369 nucleotide acid, respectively. Sequence analysis of the predicted ORF of MgEno - 1 revealed that it is of the nearly equal length and of high identity to enolase- 1 genes published in the filamentous fungi, implying that it is highly conservative, and even has the potential to serve an evolutionary scale like 18S rRNA. To our knowledge, it will be the first gene cloned from M. grisea using the technique of in silico cloning.