长筒石蒜种质资源的RAPD及ISSR研究

Study on Germplasm Resources of Lycoris longituba (Amarylliadaceae) by RAPD and ISSR

借助RAPD及ISSR分子标记对长筒石蒜的种质资源进行了初步的研究,结果表明RAPD扩增得到的77个位点中,53个位点具有多态性,约占总数的68.8%;ISSR扩增得到的67个位点中,其中62个位点具有多态性,约占总数的92.5%。因此,长筒石蒜遗传多样性是十分丰富的,可以作为育种材料储备种质资源。从UPGMA聚类图来看,依据花色区分的3种类型,被聚在一起,表明亲缘关系较近。因此,在未来的长筒石蒜种质资源利用中,不同花色的长筒石蒜可以作为品系进行开发。

Random amplified polymorphic DNA （RAPD） and inter-simple sequence repeats （ISSR） methods were used to study the germplasm resources of Lycoris longituba. Using RADP with 12 primers, a total of 77 discernible loci were obtained, of which 53 loci were polymorphic （PPB= 68.8%）. Using ISSR with 9 primers, 67 disernible loci were obtained, of which 62 loci were polymorphic （PPB = 92.5% ）. The results indicate that genetic diversity of Lycoris longituba is abtmdant, therefore, it is a good genetic material to store for breeding purpose. From UPGMA dendrograms, three Lycoris longituba types were recognized, which could be also distinguished by their flower colors. For future use, different varieties of Lycoris longituba could be cultivated.