水体中微囊藻毒素-LR的间接竞争ELISA检测

Detection of Microcystin-LR in Waters Using Indirect Competitive ELISA

在自制包被完全抗原浓度为5μg／mL、单克隆抗体工作稀释度为1：3000、酶标二抗鼠工作稀释度为1：3000、微囊藻毒素-LR浓度在0．001-30μg／L、显色底物为邻苯二胺，采用间接竞争酶联免疫吸附试验对水体中的微囊藻毒素-LR进行检测，结果表明，该方法与高效液相色谱的检测结果相关系数大于0．99，多次重复实验相对标准偏差小于10％，最低检测限能达到0．01μg／L。定量检测区间为0．01～3μg／L，该方法对[4-精氨酸]微囊藻毒素能特异性识别，对来自实际水样中的干扰有相当的耐受力．

Indirect competitive enzyme-linked immunosorbent assay （ic-ELISA） was established to detect microcystin-LR in waters, with the concentration of the complete antigen was 5μg/mL, the dilution of the monoclonal antibody was 1 ： 3 000, the dilution of the enzyme tracer （goat anti-rabbit IgG-peroxidase） was 1：3 000, the concentration range of microcystin-LR was between 0.001 -30μg/ L, and using o-phenylenediamine as substrate. The assay showed a high relativity of more than 99% with high performance liquid chromatography, a mean relative standard deviation less than 10%, a detection limitation under 0.01μg/L and quantitative detection range was 0.01-3μg/L, high specificity for [4-arginine] microcystin, and it could still perform well under the influence from the samples.