与基因AP1和LFY表达有关的蛋白质组学

The study of proteome related with the expression of genes AP1 and LFY

拟南芥分别用室温0～4℃萌发,然后培养到开花,分别从叶片和花中提取可溶性蛋白．在SDS-PAGE电泳和数字扫描分析仪分析后,选出16条明显特异的蛋白条带．同时对这些同样的样本提取RNA,以此RNA作模板,通过RT—PCR,对APl和LFY基因进行克隆,通过两个基因编码的蛋白分子量的推算,发现与16条蛋白中的第8,9条吻合．

Arabidopsis were cultivated with two kinds of treatments ： The seeds were germinating at room temperature and at 4℃, then both were cultivated to flowering. Soluble proteins were extracted from the samples of leaves and flowers while RNAs were extracted from the same samples. After SDS -PAGE, protein expression was analyzed in Digital Gel Image, 16 specific protein lines were selected to show the specific relationship between the proteins and flowering. The genes AP1 and LFY were amplified by RT - PCR. The results showed that protein lines No. 8 and No. 9 were context with genes AP1 and LFY respectively by analysed MW and appearing time.