摘要
目的探讨丁酸对牙周膜细胞增殖的影响。方法用含不同浓度丁酸(0、2、4、8 mmol/L)的培养液培养人牙周膜细胞,通过MTT法测定其生长与增殖。结果人牙周膜细胞进入指数生长期后,丁酸以剂量依赖的方式抑制人牙周膜细胞的增殖。结论龈下厌氧菌代谢生成的丁酸可能通过抑制人牙周膜细胞的增殖,进而影响牙周膜的改建和更新,促进牙周袋形成和牙周组织破坏。
Objective To determine butyrate which secreted in gingival crevicular fluid by various oral bacteria account for the growth- irhibifing activity of cultured human periodontal ligament (HPDLC) cells. Methods We therefore investigated cultured HPDLC' s growth speed in different concentrations (0, 2, 4, and 8 mmol/L) butyrate incubate solution. Results Butyrate inhibited tire growth of HPDLC in a dose dependent manner. Condusion These results suggested that butyrate released by pathogenic microorganisms can contribute to the gingival tissue dysfunction and breakdown through their actions on growth of HPDLC.
出处
《中国自然医学杂志》
CAS
2006年第2期81-83,共3页
Chinese Journal of Natural Medicine
基金
河南省杰出青年基金资助项目(项目编号:0512001000)
关键词
人牙周膜细胞
龈沟液
丁酸
Human periodontal ligament cells (HPDLE)
Gingival crevicular fluid (GCF)
Butyrate