非那雄胺与多沙唑嗪联用对鼠前列腺增生模型的影响

Effects of Finasteride Associated with Doxazosin on Rat Model of Benign Prostate Hypertrophy

目的:探讨非那雄胺与多沙唑嗪联用对鼠前列腺增生模型的影响。方法:将34只雄性W istar大鼠,随机分为模型组(28只)、假手术组(6只)。造模成功后将24只模型鼠随机分为非那雄胺与多沙唑嗪联用、非那雄胺、多沙唑嗪组、阴性对照组,继续予皮下注射丙酸睾酮4mg/d.kg造模,连续4周;假手术组继续予皮下注射等量橄榄油4周。各组实验鼠分别于注射4周后处死,取出前列腺,光镜下观察其形态学变化,计算各实验组前列腺指数(PI)。免疫组化检测bax、fas、bcl-2蛋白表达情况。结果:与阴性对照组相比,给药4周后非那雄胺与多沙唑嗪联用、非那雄胺组PI、bcl-2表达下降(P<0.05),bax、fas表达增加(P<0.05)。结论:两种药物联用显著地降低了鼠前列腺增生的进展,有望成为临床治疗前列腺增生的新方法。

Objective： To investigate effects of Finasteride associated with doxazosin on rat model of benign prostate hypertrophy. Method： 34 male Wistar rats were divided into sham operation group （ n = 6 ） and model group（ n = 28）. After rat model was successful, The other 24 model rats were randomly divided into Finasteride associated with doxazosin group, Finasteride group, doxazosin group, negative control group. The four group rats were injected subcutaneously testosterone propionate 4mg/kg. d for four weeks; sham operation group was injected subcutaneously the same dose of olive oil for four weeks. All groups were killed at 4 weeks, removed the prostates, observed the structural changes of prostates by light microscopy, calculated the prostate index （ PI ）. The expression of box,fas,bcl - 2 protein were analysed by means of immunohistochemical technique. Result： Compared with negative control group, PI, bcl -2 protein of Finasteride associated with doxazosin,Finastefide group were significantly decreased （P 〈0.05 ） , the expression of bax,fas protein significantly increased（ P 〈0.05）. Conclusion： Finasteride associated with doxazosin can induce glandular atrophy of the rat model of BPH. Applications of Finasteride associated with doxazosin therapy could be candidatod for the new treatment of BPH.