黄瓜花叶病毒2个分离物的亚组鉴定及株系分化研究

Subgrouping identification and strain differentiation of two Cucumber mosaic virus isolates

基于黄瓜花叶病毒甜菜分离物CMV-X J1和CMV-X J2独特的生物学特性,利用RT-PCR对接种寄主植物进行了检测。RT-PCR产物的RFLP结果显示,2个病毒分离物的M spI酶切图谱与CMV亚组I病毒的酶切图谱很相似,但经EcoR I酶切后出现显著差异;进一步对2个分离物的外壳蛋白基因进行了克隆,序列分析表明,CMV-X J1和CMV-X J2归属CMVIB亚组,二者存在着株系分化的趋势。

Based on particular biological characteristics of Cucumber mosaic virus beet isolates CMV-XJ1 and CMV-XJ2, detection was done by RT-PCR. RFLP of RT-PCR products showed that, the resulting fragments digested with Msp Ⅰ of the two isolates were very similar to isolate belonging to subgroup Ⅰ, but distinct difference appeared when they digested with EcoR Ⅰ. Thus CP gene of CMV-XJ1 and CMV-XJ2 were cloned. Sequence analysis revealed both were belonging to CMV subgroup IB, there existed differentiation trend between them.