碘标记神经生长因子在缺氧新生鼠脑内的吸收

Absorption of Radioiodinated Nerve Growth Factor in Hypoxic Neonatal Rats Brain

目的观察碘标记神经生长因子(125I-NGF)在缺氧新生鼠脑内的吸收情况。方法选取32只7日龄健康新生鼠随机分成实验组(125I-NGF组和125I组)及正常组(125I-NGF组和125I组),实验组制成缺氧缺血性脑损伤(HIBD)模型。分别予125I-NGF和单纯125I腹腔注射后30 min处死取脑,按如下解剖分出基底前脑、额皮质、海马、丘脑下区、小脑、嗅球、垂体,对标本进行放射性测量。结果两125I NGF组于基底前脑、小脑、额皮质、海马、嗅球均有显著吸收,且NGF在缺氧鼠额皮质、海马、小脑吸收显著高于NGF在正常新生鼠以上部位。结论外源性NGF可透过新生鼠血-脑脊液屏障,早期应用治疗HIE是可行的。

Objective To observe absorption of exogenous nerve growth factor（NGF） between normal newborn rats and newborn ratswith hypoxidosis. Methods Thirty - two healthy newborn rats（7 days of age） were divided into experiment group and normal group. Experiment rats were made into hypoxic - ischemic brain damage（HIBD） model. NGF labeled with ^125I or ^125I was injected into the rats abdominant. All of the rats were killed and brain was removed after 30 minutes. The brain was dissected into basal forebrain, forehead cortex, hippocampus, thalamus, cerebellum, smellorb, pituitary tissue samples were assayed for radioactivity in a two- channel gamma counter. Results Absorptions of two groups （^125I - NGF） were significantly higher than those of ^125I in basal forebrain, cortex, hippoeampus, cerebellum. Absorptions of hypoxic group were significantly higher than those of control group in cortex, hippocampus, cerebellum. Conclusions NGF can penertrate blood brain barrier of newborn rats into brain regions by means of peripheral administration. Early application of NGF for treating hypoxic- ischemic encephalopathy（HIE） is feasible.